منابع مشابه
Freezing and Thawing Human Embryonic Stem Cells
Since James Thomson et al developed a technique in 1998 to isolate and grow hES in culture, freezing cells for later use and thawing and expanding cells from a frozen stock have become important procedures performed in routine hES cell culture. Since hES cells are very sensitive to the stresses of freezing and thawing, special care must taken. Here we demonstrate the proper technique for rapidl...
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ژورنال
عنوان ژورنال: Genome Biology
سال: 2003
ISSN: 1465-6906
DOI: 10.1186/gb-spotlight-20030108-01